The Supernatural
The supernatural elements, primarily represented by the witches and their prophecies, play a significant role in the play. The witches' enigmatic predictions set in motion a chain of events that blur the lines between fate and free will, raising questions about the nature of destiny and the role of the divine in human affairs.

Appearance vs. Reality
Shakespeare explores the theme of deception and the contrast between appearance and reality throughout the play. Macbeth and Lady Macbeth must maintain a facade of innocence and loyalty while concealing their treacherous acts. The play also delves into the inability of characters to perceive situations as they truly are, further emphasizing the dichotomy between appearance and reality.

Guilt and Remorse
The theme of guilt and its psychological toll is prominent in "Macbeth." Both Macbeth and Lady Macbeth experience intense guilt and remorse for their actions, manifesting in hallucinations, sleepwalking, and eventual madness. Their guilt prevents them from fully enjoying the power they craved, highlighting the moral consequences of their deeds.

Gender Roles and Masculinity
Shakespeare challenges traditional gender roles and societal expectations of masculinity in "Macbeth." Lady Macbeth's ambition and cruelty defy the norms of feminine behavior, while Macbeth's hesitation and emotional vulnerability contrast with the prevailing notions of masculinity. The play explores the complexities of gender and the redefinition of true manhood.

These themes, along with others such as the corrupting nature of power, the consequences of disrupting the natural order, and the role of fate and free will, contribute to the enduring relevance and depth of "Macbeth."

The play's portrayal of the disastrous consequences of regicide and the unlawful seizure of power would have aligned with King James's views, likely making the play favorable to the monarchy.

The early 17th century was a period of intense religious belief and superstition. The depiction of witches and their malevolent influence would have been both believable and terrifying to the contemporary audience.

Shakespeare's use of poetic language, soliloquies, and the exploration of psychological depth in characters like Macbeth and Lady Macbeth would have been appreciated by the audience. These elements aligned with the evolving tastes in theater that favored complex characters and intricate plots.

While specific records of audience reactions from 1606 are scarce, the play’s immediate popularity and enduring status suggest it was well-received.

In summary, "Macbeth" in 1606 would have been perceived as a gripping and thought-provoking tragedy that addressed contemporary concerns about power, legitimacy, and the supernatural, while also showcasing Shakespeare's dramatic and poetic talents.